Maya Ibuki from Evangelion,
From
http://www.evaotaku.com/html/evafaq2.html
Q) Is Maya Ibuki a lesbian? Does she have feelings for Ritsuko?
A) Yes. Although Maya looks up to Ritsuko as her "sempai", there was clearly an intention to portray Maya's feelings towards Ritsuko as something much more complicated with deliberate gay overtones. When the phantom Rei/Ritsuko embraces Ritsuko during the film, The End of Evangelion, Maya lets a gasp of "ecstacy" escape (as described by Anno). Further, in the Newtype filmbook for End of Evangelion, Maya's final lines before she reverts to LCL are surrounded by hearts. It's clear that Gainax intended Maya to be a character with, at the very least, decisively lesbian tendancies.